Contents Menu Expand Light mode Dark mode Auto light/dark, in light mode Auto light/dark, in dark mode Skip to content
Ramona MCAM documentation
Light Logo Dark Logo
Ramona MCAMdocumentation
0.19.604
  • Getting Started
    • MCAM Usage Overview
    • Python examples
  • MCAM Workflows Manual
    • Introduction
    • Acquire an Image
    • Acquire a Z Stack
    • Acquire an XYZ Stack
    • Acquire a Video
    • Tools
      • Color Picker
      • Length Tool
      • Rectangle Tool
      • Circle Tool
      • Ellipse Tool
      • Region of Interest (ROI) Extraction Tool
      • Stack Projection
      • Stack Reduction
      • Select the GFP Channel Corresponding to the Brightfield Best Focus Slice
      • Labeling Tool
      • Binary Mask Labeling Tool
    • Recommended Model Organism Video Acquisition Parameters
    • Create a Well Alignment File
    • Compressing .nc Dataset Files
      • Single File Compression
      • Batch File Compression
    • Zebrafish Tracking
      • Acquire a Zebrafish Assay Video
      • Acquire a Zebrafish Visual Motor Response (VMR) Dataset
      • Acquire a Zebrafish Timelapse Stimulus Dataset for Acoustic Startle Response
      • Tracking Analysis
      • Animal Behavior Analysis
      • Dynamic Visual Display Module
    • Zebrafish Segmentation - Fluorescence
    • Zebrafish Morphology and Segmentation - BrightField
    • Diffuser Riser for Zebrafish Morphology and Segmentation
    • Activity Metric
      • Zebrafish Embryonic Photomotor Response (EPMR) Assay
      • Zebrafish Tail Coiling Assay
    • Best Focus Panel
    • Full Well Scan
    • Analysis
      • Cell Counting Analysis
      • Channel Threshold Analysis
      • Confluence Analysis - 4x Magnification
      • Confluence Analysis - 10x Magnification
      • Single Point Segmentation
      • Runtime Single Point Segmentation Analysis
      • Segmentation
      • Fluorescent Nuclei Segmentation
      • Runtime Segmentation Analysis
      • Tiny Organoid Segmentation and Coordinate Calculation
      • Stitching Multi-Image Well Datasets
      • Transfection Assay (Beta*)
      • Live/Dead Assay (Beta*)
    • Machine Learning
      • Model Training - Object Detection
    • Appendix A
      • Working with Nuclear Stains
  • Python API
    • Python Module
      • owl.instruments.MCAM
      • owl.instruments.Illuminate
      • owl.instruments.X_LSM_E
      • owl.instruments.MultiLidar
      • owl.mcam_data
      • owl.stitch.hugin_stitching
      • owl.stitch.hugin_global_transforms
      • owl.analysis
      • owl.color
      • owl.calibration
      • owl.visualize
    • MCAM Dataset
      • Dataset structure
      • Experiment metadata
      • Adding custom metadata
      • Saving and exporting datasets
      • Forward and backward compatibility
    • Image and Video Storage Formats
    • Color Processing
      • Grayscale conversion
      • RGB lighting color correction
      • IR (850 nm) lighting color correction
      • White lighting color correction
      • Illumination Channel ID
    • OWL Schemas
  • Web API & Command Line
    • WebMCAM
    • Command line functions
  • Data Analysis
    • Animal Tracking Data Analysis
      • Zebrafish Tracking Examples
      • Pulse Power Calibration
    • Batch Processing Tools
    • MCAM Assays
      • Activity Metric
      • Zebrafish Embryonic Photomotor Response
      • Zebrafish Tail Coil
      • Zebrafish Tracking
      • Zebrafish Flash Stimuli Calibration
      • Segmentation
    • Mask Labeling
  • Hardware & System
    • MCAM Inserts
    • Data Storage and Management
    • System Administration
    • Remote Connections to the MCAM Computer
  • Support
    • Remote Support
    • Troubleshooting
  • Legal Notices
  • MCAM Software Updates
Back to top

Appendix AΒΆ

Download

  • Working with Nuclear Stains
Next
Working with Nuclear Stains
Previous
Model Training - Object Detection
Copyright © 2017-2026, Ramona Optics, Inc.
Made with Sphinx and @pradyunsg's Furo