Zebrafish Tracking

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The Ramona MCAM supports fully automated zebrafish tracking across 96-, 24-, and custom 12-well plates in Visible and Infrared transmission illumination. 8 key-points on the body of a zebrafish are tracked as a “skeleton” as shown below:

Zebrafish tracking generates a diverse and detailed set of outputs, from raw data describing the x- and y-coordinates of tracking key-points over time to various derived metrics and visualizations, listed out and described in detail in the MCAM User Manual.

Users may use a .nc dataset file captured with the Ramona MCAM to generate zebrafish tracking outputs in 3 steps:

1. Creating a Well Alignment File - See section titled “Creating Well Alignment Files”

2. Compressing the original .nc dataset file using the generated Well Alignment File - See section titled “Compressing .nc Dataset Files”

3. Tracking the zebrafish -See below:

• Acquire a Zebrafish Assay Video
• Acquire a Zebrafish Visual Motor Response (VMR) Dataset
• Acquire a Zebrafish Timelapse Stimulus Dataset for Acoustic Startle Response
• Tracking Analysis
• Animal Behavior Analysis
• Dynamic Visual Display Module