MCAM Workflows Manual¶
Step-by-step guides for MCAM acquisition and analysis workflows. These pages are generated from the master Workflows Manual Google Doc.
The purpose of this manual is to guide users of the Multi-Camera Array Microscope (MCAM) through MCAM workflows with step by step instructions…
Capturing an image is the simplest form of data acquisition within the MCAM platform.
A z-stack is a series of images taken at different focal planes along the z-axis, capturing a 3D representation of a specimen. It’s commonly used in…
In Screening Mode with a 96-well plate it is often useful to acquire a z-stack at high resolution, however because the optical head views twenty four…
Videos can be streamed and acquired from all cameras of the MCAM simultaneously.
Available both in the MCAM Acquisition Software and the MCAM Viewer Software.
This step creates a file that will be used to detect well plate wells for analysis.
This step compresses the .nc dataset file to prepare it for analysis.
The Ramona MCAM supports fully automated zebrafish tracking across 96-, 24-, and custom 12-well plates in Visible and Infrared transmission…
The Ramona MCAM supports fully automated segmentation of zebrafish from single frame and stack datasets. This functionality can be used to define a…
The Ramona MCAM supports fully automated segmentation of zebrafish from both single-frame and stack datasets. Users can choose from seven…
The Diffuser Riser is designed to optimize imaging conditions when running experiments in Screening mode on the Kestrel. The diffuser riser provides…
Activity metric analysis provides a rapid method for computing movement in a video. This is accomplished by subtracting each pair of adjacent frames…
This feature allows users to find in-focus images for each image in the overall array.
A full-well scan allows users to scan the entire well of the well plates used in contrast to imaging a portion of wells imaged in a single-position…
Available in the MCAM Viewer Software.
Available in the MCAM Viewer Software.