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Ramona MCAM documentation
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Ramona MCAMdocumentation
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  • Getting Started
    • MCAM Usage Overview
    • Python examples
  • MCAM Workflows Manual
    • Introduction
    • Acquire an Image
    • Acquire a Z Stack
    • Acquire an XYZ Stack
    • Acquire a Video
    • Tools
      • Color Picker
      • Length Tool
      • Rectangle Tool
      • Circle Tool
      • Ellipse Tool
      • Region of Interest (ROI) Extraction Tool
      • Stack Projection
      • Stack Reduction
      • Select the GFP Channel Corresponding to the Brightfield Best Focus Slice
      • Labeling Tool
      • Binary Mask Labeling Tool
    • Recommended Model Organism Video Acquisition Parameters
    • Create a Well Alignment File
    • Compressing .nc Dataset Files
      • Single File Compression
      • Batch File Compression
    • Zebrafish Tracking
      • Acquire a Zebrafish Assay Video
      • Acquire a Zebrafish Visual Motor Response (VMR) Dataset
      • Acquire a Zebrafish Timelapse Stimulus Dataset for Acoustic Startle Response
      • Tracking Analysis
      • Animal Behavior Analysis
      • Dynamic Visual Display Module
    • Zebrafish Segmentation - Fluorescence
    • Zebrafish Morphology and Segmentation - BrightField
    • Diffuser Riser for Zebrafish Morphology and Segmentation
    • Activity Metric
      • Zebrafish Embryonic Photomotor Response (EPMR) Assay
      • Zebrafish Tail Coiling Assay
    • Best Focus Panel
    • Full Well Scan
    • Analysis
      • Cell Counting Analysis
      • Channel Threshold Analysis
      • Confluence Analysis - 4x Magnification
      • Confluence Analysis - 10x Magnification
      • Single Point Segmentation
      • Runtime Single Point Segmentation Analysis
      • Segmentation
      • Runtime Segmentation Analysis
      • Tiny Organoid Segmentation and Coordinate Calculation
      • Stitching Multi-Image Well Datasets
      • Transfection Assay (Beta*)
    • Machine Learning
      • Model Training - Object Detection
  • Python API
    • Python Module
      • owl.instruments.MCAM
      • owl.instruments.Illuminate
      • owl.instruments.X_LSM_E
      • owl.instruments.MultiLidar
      • owl.mcam_data
      • owl.stitch.hugin_stitching
      • owl.stitch.hugin_global_transforms
      • owl.analysis
      • owl.color
      • owl.calibration
      • owl.visualize
    • MCAM Dataset
      • Dataset structure
      • Experiment metadata
      • Adding custom metadata
      • Saving and exporting datasets
      • Forward and backward compatibility
    • Image and Video Storage Formats
    • Color Processing
      • Grayscale conversion
      • RGB lighting color correction
      • IR (850 nm) lighting color correction
      • White lighting color correction
      • Illumination Channel ID
    • OWL Schemas
  • Web API & Command Line
    • WebMCAM
    • Command line functions
  • Data Analysis
    • Animal Tracking Data Analysis
      • Zebrafish Tracking Examples
      • Pulse Power Calibration
    • Batch Processing Tools
    • MCAM Assays
      • Activity Metric
      • Zebrafish Embryonic Photomotor Response
      • Zebrafish Tail Coil
      • Zebrafish Tracking
      • Zebrafish Flash Stimuli Calibration
      • Segmentation
    • Mask Labeling
  • Hardware & System
    • MCAM Inserts
    • Data Storage and Management
    • System Administration
    • Remote Connections to the MCAM Computer
  • Support
    • Remote Support
    • Troubleshooting
  • Legal Notices
  • MCAM Software Updates
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Legal Notices¶

Copyright Notice¶

Copyright © 2017-2026, Ramona Optics, Inc. The information herein should aid in the development of software associated with the Ramona Optics MCAM™.

The Ramona Optics MCAM™ is provided for use by the use “AS-IS” and any express or implied warranties, including but not limited to, the implied warranties of merchantability and fitness for a particular purpose are disclaimed.

You should have received a license detailing your rights associated with this software an any other third party software bundled with the owl module. Otherwise, you are using unlicensed software.

Patent Notice¶

The MCAM Hardware and software are covered by the following patents

  1. US Patent No. 10976535: Multiple camera microscope imaging with patterned illumination.

  2. US Patent No. 11525997: Multiple camera microscope imaging with patterned illumination.

  3. US Patent No. 11153508: Unscanned Optical Inspection System using a micro camera array.

  4. US Patent No. 11415791: Snapshot polarization imaging with a micro-camera array microscope.

  5. US Patent No. 11353691: High-speed imaging with a subsampled array of image sensors.

  6. US Patent No. 11477410: Method to capture multiple simultaneous microscopy images from multiple digital sensors using field programmable gate arrays.

  7. US Patent No. 11477411: Method to capture multiple simultaneous microscopy images from multiple digital sensors using field programmable gate arrays.

  8. US Patent No. 11487097: System and method for synchronized fluorescence capture

  9. Patent Pending

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